Pfizer and Moderna mRNA Vaccines Attack Bone Marrow Stem Cells and Drastically Alter Gene Expression
Researchers are also using LNPs/mRNA for gene editing. Three studies reviewed.
Source: Global Research
Pfizer and Moderna mRNA Vaccines Attack Bone Marrow Stem Cells and Drastically Alter Gene Expression
Researchers are also using LNPs/mRNA for gene editing. Three studies reviewed.
Global Research
I review three recent papers:
Sep. 7, 2023 – Zurlo et al – The anti-SARS-CoV-2 BNT162b2 vaccine suppresses mithramycin-induced erythroid differentiation and expression of embryo-fetal globin genes in human erythroleukemia K562 cells
Jul. 27, 2023 – Breda et al – In vivo hematopoietic stem cell modification by mRNA delivery
Jan. 22, 2023 – Puccetti et al – Biodrug Delivery Systems: Do mRNA Lipid Nanoparticles Come of Age?
Sep. 7, 2023 – Zurlo et al – The anti-SARS-CoV-2 BNT162b2 vaccine suppresses mithramycin-induced erythroid differentiation and expression of embryo-fetal globin genes in human erythroleukemia K562 cells
Italian researchers treated K562 stem cells with increasing concentrations of Pfizer COVID-19 mRNA vaccine
What are K562 stem cells? – K-562 are lymphoblast cells isolated from the bone marrow of a 53-year-old chronic myelogenous leukemia patient. The K-562 cell line is widely used in immune system disorder and immunology research.
What are lymphoblast cells? Immature white blood cells that develop into healthy immune cells called lymphocytes. In leukemia, lymphoblasts don’t mature, instead they multiply rapidly in bone marrow and interfere with all blood cell production.
researchers were able to inhibit the growth of stem cells as Pfizer dose increased
Spike protein levels also increased with higher Pfizer mRNA doses
Spike protein increased expression of pro-inflammatory genes through up-regulation of NF-kB
Spike protein drastically decreased expression of several globin genes
Spike protein suppressed erythroid differentiation of stem cells
“The impact of SARS-CoV-2 Spike protein on cellular functions is of key interest”
“searching for circulating Spike in plasma of COVID-19 patient might help in understanding unexpected adverse effects following COVID-19 mRNA vaccination”
Conclusion: “SARS-CoV-2 S-protein, COVID-19 mRNA vaccines and SARS-CoV-2 infection might have dramatic effects of the hematopoietic compartment”
Conclusion: “need of great attention on possible alteration of hematopoietic parameters following SARS-CoV-2 infection and/or COVID-19 vaccination”
Spike protein production rises dramatically with increasing doses of Pfizer mRNA vaccine. This rise appears exponential.
Increasing doses of Pfizer mRNA vaccine cause dramatic suppression of globulin gene expression in bone marrow stem cells
Jul. 27, 2023 – Breda et al – In vivo hematopoietic stem cell modification by mRNA delivery
This paper was published in “Science”
Summary: NIH funded authors injected LNPs/mRNA and delivered them to bone marrow stem cells where they conducted gene editing and “bone marrow transplantation”
The researchers developed two payloads: one that edited a mutation for sickle cell disease, and another that selectively killed hematopoietic stem cells, which would eliminate the need for chemotherapy before HSC transplantation.
If the therapies can be successfully adapted to people, this approach “will actually make gene therapy affordable, not only to our patients but also to our health care system,” says Hamideh Parhiz, a biotechnologist at the University of Pennsylvania, who co-led the research.
The researchers designed the lipid nanoparticles to target HSCs using an antibody that binds to the protein CD117, which is found on these cells’ surface.
After confirming that the nanoparticles were breaking through into about half of blood cells, they loaded the antibody-coated nanoparticles with an mRNA encoding a protein that induces cell death.
Although the nanoparticles killed HSCs, the researchers discovered some off-target effects, so they added tiny bits of noncoding RNA that kept the protein from killing other cells. “That’s when we got success,” Parhiz says.
In another experiment, the researchers stuffed their nanoparticles with an mRNA sequence that produces a gene editor when it enters the cell. The editor targets a mutation in hemoglobin causing sickle cell disease.
The researchers tested the gene-editing nanoparticles on cells grown from samples taken from people with the disease. Reversing the mutation resulted in more than 95% of blood cells taking on a typical round shape rather than the sickle-like appearance characteristic of the disease.
Parhiz and her colleagues are working on fine-tuning the approach and testing it further in animals to get a better understanding of how efficiently it edits intended genes and how well it targets HSCs.
The study is “an impressive advance,” says David R. Liu, a chemist and gene editing expert at the Broad Institute of MIT and Harvard. Though many steps remain before clinical testing, he says, the approach “could lay a foundation for the much broader availability of programmable therapeutic gene editing to treat a variety of genetic blood disorders”
“A step toward stem cell engineering in vivo”
Journal Intro: Hematopoietic stem cell (HSC) gene therapy provides lifelong and substantial benefits for several life-threatening inherited diseases, such as primary immunodeficiencies, storage disorders, and hemoglobinopathies.
Currently, HSC gene therapy requires harvesting large numbers of a patient’s hematopoietic stem and progenitor cells (HSPCs), which undergo gene transfer or editing ex vivo. Before infusion, the cell product is qualified to ensure that it meets rigorous safety and efficacy standards, and the patient undergoes conditioning chemotherapy to deplete endogenous HSPCs and make space for the engineered cells to engraft in the bone marrow.
However, the need for laborious manufacturing and the toxicity associated with the conditioning regimen limits the broad application of these treatments.
On page 436 of this issue, Breda et al. provide a proof of principle of in vivo genetic engineering of HSPCs in the bone marrow of mice by leveraging transient delivery of mRNA through lipid nanoparticles (LNPs) functionally coupled to antibodies that target HSPCs.
Jan. 22, 2023 – Puccetti et al – Biodrug Delivery Systems: Do mRNA Lipid Nanoparticles Come of Age?
“Although recent research has largely focused on advancing mRNA vaccines and large-scale manufacturing capabilities, the technology has also been used to develop various immunotherapies, gene editing strategies, and protein replacement therapies.”
“Lipid nanoparticles (LNPs) have emerged as a very promising delivery method. However, when intravenously delivering LNPs, most of the cargo is trapped by the liver”
Modifying the composition of the lipids in LNPs allows for the more specific delivery of the LNPs to some organs
“Messenger RNAs (mRNAs) present great potential as therapeutics for the treatment and prevention of a wide range of human pathologies, allowing for protein replacement, vaccination, cancer therapy, and genomic engineering”
mRNA for vaccines: “Optimal vaccine targets can be quickly discovered through genetic sequencing, rapidly yielding templates for subsequent large-scale mRNA production. The rapid discovery process, synergistically paired with relatively inexpensive biomanufacturing costs for LNP formulations, have enabled mRNA vaccine candidates to reach clinical testing and receive regulatory authorization much faster than traditional vaccines.”
Both Pfizer & Moderna mRNA vaccines “contain nucleoside-modified mRNAs that induce the membrane-bound expression of a perfusion-stabilized, full-length SARS-CoV-2 spike protein. In each case, the mRNA vaccines were formulated using LNPs for intramuscular injection. The rapid development and potent efficacy of these vaccines will serve as a strong benchmark for the advancement of future mRNA-based vaccines against a broad set of diseases.”
“to increase the efficacy of mRNA-based vaccines, additional strategies such as self-amplifying mRNA vaccines are being developed.
Self-amplifying mRNA vaccines use an engineered RNA virus genome in which the genes for the antigens of interest are inserted in place of those encoding the virus structural proteins while the genes for the virus RNA replication machinery are kept intact.
In contrast to traditional mRNA-based vaccines, self-amplifying mRNA vaccines allow for the intracellular replication of antigen-encoding RNA, resulting in a higher level of antigen production that enhances vaccine efficacy.
Self-amplifying mRNA vaccines show some difficulties compared with mRNA vaccines.
They have a necessarily higher molecular size due to the presence of the viral-derived genes for the RNA replication machinery, which can also cause immunogenicity, thus limiting their potential repeated use
Thus far, the self-amplifying mRNA vaccine platform has been applied against diverse viruses including influenza, Ebola, hepatitis C, rabies virus, Toxoplasma gondii, human cytomegalovirus, and HIV-1.
mRNA for Gene Editing: “In addition to protein replacement and vaccines, more recently, the development of CRISPR (Clustered Regularly Interspaced Short Palindromic Repeat) technology led to the application of mRNAs in gene editing and extended their use in pathologies requiring not only protein expression but also gene knockout”
My Take…
We know from the Japan biodistribution study obtained by Virologist Dr.Byram Bridle, that Pfizer COVID-19 mRNA vaccine accumulates in the bone marrow.
On May 27, 2021, Moderna’s Fourth Annual Science Day, Moderna boasted about their ability to deliver mRNA to the bone marrow, causing “long term modulation of all hematopoietic lineages” on page 113 of a document that is now impossible to find (click here):
Key points from Zurlo et al paper:
Pfizer COVID-19 mRNA vaccine accumulates in the bone marrow and can inhibit the growth & suppress the differentiation of bone marrow stem cells
Pfizer spike protein can drastically alter gene expression in stem cells
Pfizer spike protein can increase expression of pro-inflammatory genes
spike protein production in bone marrow stem cells increases dramatically with increasing mRNA dose (looks exponential)
authors conclude: “Pfizer spike protein might have dramatic effects on the hematopoietic compartment”
Key points from Breda et al and Puccetti et al papers:
LNPs/mRNA can be delivered to bone marrow stem cells where they conduct gene editing and bone marrow transplantation
LNPs can be modified via a surface “decoration” to improve targeted delivery of mRNA cargo
mRNA can be encoded with a protein that induces bone marrow cell death
mRNA can also be encoded with sequence that produces a “gene editor” when it enters the cell
LNP/mRNA is repeatedly referred to as “gene therapy” and a platform for “genetic engineering” including “gene editing strategies”.
My Concerns…
All these recent papers downplay the dangers of the LNP/mRNA platform and completely ignore the millions of COVID-19 mRNA vaccine injuries & deaths, pretending they are not happening as they push forward
COVID-19 mRNA vaccines are being referred to as a “resounding success” even though they are a complete failure.
Pfizer COVID-19 mRNA vaccine messes with bone marrow stem cells, affects their growth and differentiation, the clinical implications of which we don’t understand. Can this lead to turbo cancers such as leukemias?
Pfizer COVID-19 mRNA vaccine alters gene expression in bone marrow stem cells the clinical implications of which we don’t understand.
Spike protein production in stem cells is not linear – slightly more mRNA can lead to exponentially higher spike protein production – may partly explain severity of COVID-19 mRNA vaccine injuries in someone who may have received only slightly higher concentration of mRNA in their vaccine dose.
LNP/mRNA is a gene therapy, and a platform for “genetic engineering” including “gene editing strategies”.
Slight modification of LNP’s external “decoration” can drastically impact where LNPs get delivered. Researchers are already playing with these modifications.
LNP/mRNA technology is being combined with CRISPR tech for gene editing.
researchers are playing with “self-amplifying mRNA”, which means that the mRNA will now be able to replicate itself within YOUR cells so you get exponentially higher levels of spike protein produced to “improve vaccine efficacy”. As if we all need EVEN MORE spike protein.
Dr. William Makis is a Canadian physician with expertise in Radiology, Oncology and Immunology. Governor General’s Medal, University of Toronto Scholar. Author of 100+ peer-reviewed medical publications.
Source: MarkTaliano.net
Malice Aforethought
by Dr. Mike Yeadon
A message written to a trusted campaigning friend, who is struggling to accept that what is happening is intentional. It is indeed a big step to move from justifiable criticism of presumed incompetent politicians and industrialists to putting the pieces together in such a way that they can point only to intentionality. See what you think.
Best wishes
Mike
Dear X,
I find that attempting to put all the information together in such a way as to allow for the whole even to be benign or at least not wholly malevolent is impossible.
That’s because, as you’ve no doubt heard me say, I believe it is malevolent!
I too struggled early on, arguing “surely they must have known this!?” Etc.
Once I allowed for the possibility that all the bad things were intentional, I find all the rest falls into place. Obviously that alone doesn’t prove that it was intentional.
Some early clues to intentionality are the coordinated responses of scores of governments to the alleged pandemic: lockdowns, masking, mass testing of the well, the misapplication of PCR-based techniques to bulk testing of clinical samples, selective business & school closures, border restrictions etc.
Not a single country had any of this as a core part of their own pandemic preparedness plan.
Even the WHO’s scientific evaluation of NPIs concluded none of them worked & the only changes worth a dime were asking those with symptoms to remain at home until recovered & to increase the frequency of hand washing (because the route of transmission wouldn’t initially be known for sure).
I argued at the time that the only way all the countries could have adopted all these useless yet damaging & costly NPIs is if there was supranational coordination.
Whether that from WHO, WEF etc I don’t know. But illegitimate in any case.
We now know that they knew that imposing these restrictions would save nobody, yet the negative consequences would be devastating, even lethal for some, who would no longer have access to the medical care they needed. Additionally, the use of furlough was obviously going to be enormously damaging to sovereigns who were already borrowed to the hilt.
I note the widespread adoption of an American term, furlough, into public discourse. In U.K. we never previously used the term. Nobody remarked on its arrival, which telegraphed the leading role played by Americans.
Then we have the imposition of radically altered medical protocols.
Because of my long exposure to matters respiratory, I knew immediately they began panicking about needed 30,000 mechanical ventilators that something truly demonic was at hand. It’s never appropriate, in a patient with an unobstructed airway & an intact chest wall to sedate, intubate and ventilate them. Mechanical ventilation is certainly a marvellous, life-saving thing, but it comes with serious risks to the frail patient, in the form of ventilator acquired pneumonia, lung injury from use of pressure to inflate the lungs and more. The appropriate treatment would be an oxygen mask, single, low dose benzo, a cup of tea and a biscuit and a caring hand upon an arm.
In the USA as well many, once in this vulnerable state, were given remdesivir & not given total, intravenous nutrition. In most cases it was just a matter of time before they died.
In care homes, there was indiscriminate use of inappropriately high doses of midazolam and morphine. Not only high doses but repeatedly given to their elderly charges. They’d been told to do it by the highest medical authorities in the land and so few questioned it.
My PhD by coincidence was in this area, the effect of opiates on respiratory function. The discovery of multiple opiate receptors raised the possibility that it might be possible to invent receptor selective ligands that would relieve pain with reduced respiratory depression. Unfortunately, both are mediated primarily by mu opiate receptors, both centrally and in the periphery.
The combination of opiate agonists and benzodiazepines is contraindicated in patients unless close monitoring (for signs of respiratory depression) was in place.
It isn’t & cannot be in a care home. They too were murdered in large numbers.
Finally, in the community, the family doctors were cautioned not to prescribe antibiotics in cases of covid “because antibiotics cannot treat viral illnesses”. It’s well known that what is usually termed secondary bacterial infection is generally what actually leads to death in this situation. However, the records show that prescriptions for antibiotics for suspected bacterial infection of the lungs fell by 50% & large numbers of people died avoidable deaths (and rather horrible deaths, too).
It’s not possible to regard all of this evidence without concluding that malice aforethought was at work here. It’s literally diabolical, what they did.
How it was done with so little pushback still confuses me. I do know that from the late-1990s right through late-2019, there was a sequence of tabletop simulations of global pandemic scenarios & bioterrorism scenarios, which allowed the perpetrators to hone their craft in the responses & control measures imposed. I believe some of these were translated onto the ground, giving emergency response team a chance to form & to rehearse what most of them thought were appropriate, given the fictional set up, though this is speculative.
Then we come to the “vaccines”. Given a career in pharma and biotech, I knew that it was impossible to create a vaccine in under 5-6 years if they were going to demonstrate clinical safety and hone manufacturing to yield the customarily high quality manufacturing necessary to produce tightly defined final drug product. If the latter if not done, it’s pointless doing the former, because what would otherwise be injected wouldn’t be what had been used in the clinical trials.
In other words, if there was a need for a new vaccine, you would never even contemplate running such a program, because no pandemic in history lasts a fraction of the minimum time necessary to create a safe and effective new vaccine.
Yet they went ahead. This too is malevolent, let alone the extraordinary lying, censorship & smearing of dissenters.
Given my entire career used “rational drug design” principles to design and test molecules, I was able to put myself in the shoes of the designers of the jabs.
There are several, completely obvious safety issues built into these products.
One is the axiomatic induction of “autoimmune” responses, regardless of which antigen was selected.
The next was choice of antigen, where no one would pick spike protein, because it was highly likely to be directly toxic, it’s subject to the most rapid mutation (so a vaccine might lose efficacy) & also it’s the least different from human proteins (and so might trigger bystander attacks on even somewhat similar self proteins).
Yet all four leading players chose this antigen. What a coincidence! I’d have called up my peers in the other companies to endure we didn’t do that. That’s because it would be highly undesirable to have common risks to all programs.
On formulation, the mRNA-based products both selected LNPs to encapsulate their message. Yet there was industry knowledge that these not only travel all over the body including into the brain but that they accumulate in the ovaries.
Yet, knowing this, the companies & regulators went ahead and then others compounded the toxicity risk by recommending these injections in pregnant women and children.
I was still slow to piece together all this evidence of carefully thought out harms. But eventually I got there are have been speaking in what many regard as extreme terms ever since.
I’m afraid there’s no hiding from the reality that this is a global coup d’etat and intentional mass murder.
Worse, we see the encroachment of surveillance technology and legal powers to introduce digital ID & CBDC as well as to eliminate cash.
It’s not difficult to come up with scenarios where presentation of digital ID will become mandatory.
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